The show, named 'The Perfect Machine', will arrive at the Summerlee Museum of Scottish Industrial Life this weekend to celebrate 100 years of Scottish cycling history.
There will be a mix of donated cycles, photographs, audio and visual material, as well as an array of artefacts and memorabilia on display at Summerlee until the end of August.
The event comes ahead of North Lanarkshire's involvement in this summer's Games, when it will host events including the Cycling Time Trial.
The race will travel through Stepps, Chryston, Gartcosh and Muirhead, and will see Olympian and Tour de France winner Bradley Wiggins compete.
Among the exhibits on show at the exhibition is record-breaking Scottish cyclist Mark Beaumont's back-up bike, which he used for his long-distance cycle through America, Alaska and Chile.
Another famous Scot has donated to the collection.
Stunt cyclist Danny McAskill has given one of the jump-bikes he uses for his extreme shows and videos.
One of the bikes used in Emirates Arena and the Sir Chris Hoy Velodrome will also be on display.
Alongside the elite bike a time-lapsed film will be premiered to show how the velodrome was built.
Glasgow Museums have also contributed to the show, donating various bike collections and trophies from Team Scotland's illustrious cycling history.
Among the more unusual exhibits on display will be a bicycle created at the Govan-based Wooden Bike Project.
The project allows Glaswegian designers to create one-off bicycle frames from recycled material.
Police Scotland officer PC David Wandrum has also donated a bike he has used while on community patrols.
David, who is based in Coatbridge, has been a policeman for 12 years and a community officer for the past six.
He said: "I use a police bike as my primary mode of transport, regardless of the time of day and no matter what the weather is.
"Every police officer using a cycle has undergone an extensive training course, ensuring cycling competence on a wide range of road surfaces and types of cycling conditions.
"It is not unheard of for officers to walk both their cycles and the arrested person back to the station."
Exhibition curator Justin Parkes said: "This has been a most fascinating exhibition to develop.
"A host of memories and memorabilia have come in since our appeal for cycling stories back in the spring.
"As word spread, we have also amassed a wonderful collection of fascinating cycles from clubs and private donors.
We are grateful to everyone who has contributed and help make this a 'must see' exhibition during the |Games period."
Chairwoman of CultureNL, Heather McVey, said: "Summerlee has pulled out all the stops to create this superb exhibition to coincide with the 2014 Commonwealth Games.
"With the road racing in the north area and the triathlon in Strathclyde Park, North Lanarkshire is now firmly on the Games map.
"The Perfect Machine makes a fascinating extra contribution to visitors' see-and-do lists, as well as for our own residents, and cyclists or non-cyclists are all welcome."
Summerlee, which is built around a restored ironworks, welcomes upwards of 150,000 visitors a year.