The Fit in 14 campaign encourages employees to make small changes in their working day, such as taking the stairs or going for a walk at lunchtime.
The Scottish Government hopes the Glasgow Games will inspire people to be more active and create a healthier nation in the long-term.
It has published a 10-year physical activity action plan in consultation with NHS Health Scotland, highlighting that inactivity results in around 2,500 premature deaths in Scotland and costs the NHS around £91 million each year.
Launching the campaign at an Asda store in Dumbarton, Commonwealth Games and Sport Minister Shona Robison said: "The Scottish Government is committed to increasing physical activity and we want to make Scotland a more active country by encouraging people to make physical activity a part of their everyday lives.
"It is well known that regular activity provides a great range of health benefits and makes people feel happier, less stressed and can add years of quality life.
"The benefit of the Fit in 14 campaign is that we don't expect people to run a marathon or join a gym, however small measures like taking the stairs instead of the lift, or going for a walk during lunch break, are great steps forward towards a healthier lifestyle.
"Furthermore, having a physically active workforce can also lead to 27% fewer sick days, an average saving of £135 per employee. It also reduces workforce injuries and accidents by 25%."
Asda, with more than 20,000 employees, is the first company in Scotland to pledge its support to the campaign.