The indie-rock group's hit songs, including The Woodpile, will be played more than 100 times a day on Hard Rock's in-cafe video system throughout September.
The songs will feature on 2500 screens in Hard Rock's 138 cafe locations in 54 countries around the globe.
Scott Hutchison, lead vocalist and guitarist of Frightened Rabbit, said: "Music is meant to be shared.
"That's why we started Frightened Rabbit.
"We are honoured to have the opportunity to connect with new fans around the world inside Hard Rock Cafes."