John Cleese, Terry Gilliam, Eric Idle, Terry Jones and Michael Palin from the comedy troupe have sold off places to appear alongside them during their string of performances at the O2 Arena in London.
The announcement of the charity figure comes as it is reported the stars of the shows will rake in more than £2 million each for the shows, plus the income from cinema screenings and a TV broadcast.
The Monty Python Live (Mostly): One Down, Five To Go shows have seen the surviving stars performing live together for the first time in 30 years.
Fans have been given the chance to bid for their guest role in the classic "Bruces" sketch .
The Mercury Phoenix Trust, Shelter, the Roundhouse, Maggie's Centres and the NSPCC were chosen by the reunited team to receive money from the first five nights earlier this month.
The Stammering Centre, GOSH Charity, the Dispossessed Fund and Nordoff Robbins will benefit from the second batch of shows which are taking place this week.