Remploy's Springburn site is to end production after no viable bidders for the business, which produces wheelchairs for the NHS, came forward.
Around 41 workers will leave today with eight remaining to wind down the factory before it closes its doors for good at the end of March.
Firms are being offered a cash incentive by the Scottish Government to take on redundant Remploy workers.
Is is unclear how many out of the 49 workers have secured other posts.
The site was one of the factories announced for closure in March last year after being assessed as not commercially viable.
It lost £187,000 in 2011, according to the Department for Work and Pensions.
Workers at factories in Aberdeen, Edinburgh and Wishaw were also told their sites are among 36 which are to be closed in the UK.
Remploy announced in October last year that it was in talks with R Link Ltd in a bid to keep the factory open, but no sale could be agreed.
Enterprise Minister Fergus Ewing said the situation had been "poorly handled" by the UK Government from the outset and the closures were a travesty.
Phil Brannan, GMB union official at the Springburn site, said the closure decision was "a disgrace".
He said: "The whole process has been an absolute disgrace from the beginning. Staff here feel extremely angry."
A spokesman for Remploy said: "The 41 employees who haven't found jobs will go into a government support package with a case worker assigned to them."