Jane Burns, 48, from Balornock, ran the brothel from a flat in Berkeley Street, in the city centre.
In October last year at Glasgow Sheriff Court, she pleaded guilty to managing, or acting or assisting in the management of, a brothel.
After an investigation, police discovered that £137,115 of her income, in the six years leading up to her arrest, could not be accounted for legitimately.
The confiscation order for £69,115 has been granted at Glasgow Sheriff Court.
During a previous hearing, the court heard how police received information that a brothel was being run from the flat.
Officers searched the property on March 3, 2011 and found Burns in the bedroom with a naked man.
She told police she worked as a masseuse and was there to do a massage.
However, items and documents recovered from the flat suggested it was being used for illegal brothel keeping.
The flat is now being repossessed.
Lindsey Miller, head of the Serious and Organised Crime Division, said: "Brothel-keeping, like any other criminal offence which makes money, is subject to the powers of the Proceeds of Crime Act.
"In this case, the court classed Burns' brothel-keeping as a 'lifestyle' offence, enabling us to look at her finances over the six years leading up to her arrest.
"Our financial investigators found that £137,115 of Burns' income could not be accounted for legitimately.
"I am satisfied that today's confiscation order for £69,115 represents the amount available to us at this time.
"This money will be added to the £60million already gathered from Proceeds of Crime and will be re-invested by Scottish Ministers through the CashBack for Communities programme."
Burns will be sentenced in November.