The walkers, dressed in bright purple T-shirts and carrying purple balloons, took part in the city's first seven-mile Bridge Walk hoping to raise £50,000 for Kidney Research UK.
They started at the Riverside Museum at 10am before heading south over the Millennium Bridge and then back north over the Clyde Arc, nicknamed the Squinty Bridge.
Fundraisers then made their way over the Tradeston Footbridge - the Squiggly Bridge - before heading back over the King George V Bridge.
The walk also took in the Jamaica Street, Victoria, Albert and bridges, as well as the suspension bridge, before returning, via the Millennium Bridge, to the finish back at the Riverside Museum.
Sandra Currie, chief executive for Kidney Research UK, said she was "thrilled" with the turnout.
She said: "It was brilliant. There were purple balloons and purple-clad people everywhere. There was a lovely atmosphere.
"People enjoyed seeing Glasgow from a different view, it was nice to look up and down the river. You don't see it on the bus or driving.
"It gave us all a different perspective on Glasgow."
The organisers will find out this week how much they have raised and it will go to fund research projects that are looking into treatments for and the causes of kidney disease.
Around 4300 people in Scotland are receiving treatment for kidney failure, 1500 of them in Glasgow.
A similar event has been run in London for a number of years, but this was the first Bridge Walk in Glasgow.
Ms Currie said: "We wanted to raise money to fund research and get people talking about the work that we do and understand it.
"We had a lot of kidney patients there and the family and friends of people with kidney disease.
"For so many people to turn out at the first event in Glasgow and to have so many people saying they want to come back next year is better than we could have hoped.
"It was fantastic."