The Glasgow Comedy Festival show, called PPOWH!, was held at the city's King's Theatre to raise money for the hospice and its Brick by Brick campaign, which is backed by the Evening Times.
A host of high-profile performers treated the audience to their best lines, with a line-up that included Fred MacAulay, Des Mclean, Janey Godley, Scott Agnew, Eddi Reader, Michael Redmond and Raymond Mearns.
Evening Times columnist Michelle McManus also made an appearance and topping the bill was Irish stand-up comedian, Dylan Moran, star of cult comedy show Black Books.
Compering last night's event were DJ and stand-up Des Clarke and Chewin' the Fat comedian Karen Dunbar.
Karen said: "I always love working with the King's Theatre, I should get a mortgage on the dressing room I'm in so much, but to be involved with such a great fundraiser as the hospice Brick by Brick campaign is just tremendous.
"I've been working closely with them for six months, so I'm really happy to be doing the show.
"It's such a great line-up as well, and to be hosting with Des, he's like my wee brother, now that we've done the panto together.
"I can't believe how Glasgow's International Comedy Festival has grown."
The 2013 festival chose the The Prince & Princess of Wales Hospice as its nominated charity.
Proceeds will help the appeal to raise £15million to build a hospice on a site by Bellahouston Park.
Event organiser and corporate fundraiser for the hospice, Cassie Oliver, said: "We are proud to have been selected as the charity partner for this year's Glasgow International Comedy Festival Benefit night.
"Humour plays an important role in the work that we do at the Hospice, so this event is a perfect fit in helping us raise much- needed funds."