Last week, councillors considered a report suggesting charges be introduced in a bid to raise cash.
It revealed the council's land and environment department, which is responsible for rubbish collections, had to make savings of more than £7 million by the end of March.
The report suggested one way of saving money would be to charge for bulk uplifts of rubbish, which are presently free.
Councillors agreed to look at the proposal and were due to get a more detailed report in November.
But council leader Gordon Matheson has ruled out the plan, insisting introducing charges is not an option.
He said: "There will be no charges introduced for bulk refuse uplift in the city.
"I know just how valuable this service is to people in communities across Glasgow and they can be assured that they won't have to pay for it.
"Asking people to pay for throwing out large items of household rubbish is not an option for the city council. It would simply be the wrong thing to do.
"Bulk refuse uplifts also play an important part in the Clean Glasgow campaign, which is tackling the wider problems of litter, graffiti, fly- tipping and dog fouling.
"The campaign has made a real difference as far as these complaints are concerned but everyone has to take responsibility for their actions.
"By providing a free and efficient bulk refuse uplift service to households we are specifically helping reduce fly-tipping.
"That is another reason why the council will not be introducing charges for this service."
City residents can arrange to have large items of rubbish uplifted by the council free of charge.
Payment for uplift is only in place for builders' materials, old sheds and outbuildings, tree trunks, soil, windows and cast iron baths.
Councillors were told last week that during the past three years, a significant number of employees have left the land and environmental services department.
The report read: "We will face considerable challenges to maintain and improve service levels with less staff."
A council spokesman said it was common knowledge the local authority has had to make substantial savings across all services because of the huge reduction in funding from the Scottish Government.
He added: "In spite of the challenges the department faces, ambitious targets have been set to improve the city's approach to energy, waste and transport.
City roads bosses hope to raise an extra £376,000 by increased bus lane camera enforcement and an added £1 million by raising on-street parking charges.