Residents and workers say parking in Shawlands is "horrendous" because of limited spaces - and now they will not be able to park on the main road.
Temporary parking restrictions will be in place on Kilmarnock Road, between Millwood Street and Skirving Street, from Monday until April 26.
The parking ban, which will also hit visitors and shoppers, is being brought in by Glasgow City Council, while workers widen the pavement.
A section of pavement will be broadened by two metres and laid with a new granite surface and new street lighting.
One local resident, said: "Parking in Shawlands is already horrendous, I can't imagine it getting much worse.
"But there's no doubt a 15-week ban will make it almost impossible.
"I understand the council want to improve the pavements, but surely there must be a more efficent - and quicker - way to do it."
Work will start on Monday and last for three and a half months. It is the first phase of public realm improvements by the city council on Kilmarnock Road.
A traffic notice issued by the local authority said no one could "load or park any motor vehicle" on the section of the busy street.
A council spokesman said: "Temporary no waiting and loading restrictions are scheduled to be in place on Kilmarnock Road for 16 weeks.
"This will enable pavement widening to be carried out between Walton Street and Mount Stuart Street with the existing surface being replaced by granite.
"The road between Milwood Street and Skirving Street will also be resurfaced."
Similar parking bans have also been put in place for sections of George Street, in the city centre, and Saracen Street in Maryhill.
Council bosses recently came under fire for increasing the price of parking for an hour on Kilmarnock Road by 300%.
Since December 6, drivers have had to pay 80p for an hour of parking instead of the previous 20p for an hour.
During the last financial year, city motorists paid out £8.7million in on-street parking charges.
The latest figures reveal that on-street parking charges have generated an average daily income for Glasgow City Council of £23,826.
At the time, a Glasgow City Council spokesman defended the huge increase in charges.
He said: "Parking charges in many areas of Glasgow, such as Kilmarnock Road, have not increased since 2004."
Local business owners and shop keepers have since launched a petition calling on the local authority to reverse the increase.