More than 100 works will be on display, including The Singing Butler, which sold at Sotheby's in 2004 for almost £750,000 - a then record-breaking price for a work by a Scottish artist sold at auction.
The exhibition will also feature the works Dance me to the end of Love, Bluebird at Bonneville and The Billy Boys.
Visitors to Kelvingrove will also see a broad range of Vettriano's less famous paintings including erotically charged night-time interiors, self portraits and special commissions.
There will also be works created for the Bluebird Club celebrating Sir Malcolm Campbell's land-speed records and others to mark the centenary of the Yacht Club of Monaco's world-famous yacht Tuiga.
The exhibition, which opens on Saturday September 21 and runs until February 23, will be the most comprehensive exhibition ever devoted to the artist.
Some of the works have not been seen for more than 20 years, are being loaned by famous owners from around the world, including actor Jack Nicholson.
Self-taught artist Mr Vettriano left school at 16 to work as a mining engineer.
He told the Evening Times: "I was deeply touched when approached by Kelvingrove Art Gallery about staging a retrospective exhibition for me.
"I look forward to spending some time in Glasgow and renewing my acquaintance with some of my favourite paintings in Kelvingrove's collection."
A Glasgow Life spokesman said: "We expect the exhibition to be very busy."
Tickets, are available to buy from the Kelvingrove shop, cost £5 (£3 conc), with under 16s admitted for free.
To book by phone, call 0141 353 8000. There is a booking fee of £1.50 for each transaction and to book online go to www.glasgowlife.org.uk/museums.