The X Factor judge and Take That star will perform at the event on Friday, December 13 as Clyde 1 celebrates its 40th birthday.
Barlow has sold more than 50 million albums worldwide and won six Ivor Novello awards.
As well as discovering the latest pop talent on X Factor, Gary has been working hard in the studio on his first solo album for 14 years, Since I Saw You Last, which is due out on November 25.
He will be performing classic hits alongside new material and will share the stage with a host of A-list pop acts, including The Saturdays, McFly, Union J, Rizzle Kicks, Little Mix, Naughty Boy, John Newman, Shane Filan, Tom Odell and Neon Jungle.
The event will be the biggest ever hosted by the station, with more than 12,000 fans expected to pack the Hydro.
Clyde 1 DJ George Bowie said: "Britain has produced many pop superstars, but they don't come much bigger than Gary Barlow."
Tickets can be bought at ticketSOUP.com or by calling 0844 395 4000.