Lisa McCabe, 31,and her team from Stardust Dog Walking, will appear in an advert as part of a special showing at the Cineworld in Renfrew Street - Scotland's largest cinema.
Last week Lisa, who was featured in the Evening Times' Glasgow Means Business column, scooped a gong at business accelerator Entrepreneurial Spark's Hatchery Hits The Street awards.
Now her team will hit the big screen in the 30-second advert coinciding with the showing of the first episode of the BBC documentary The Entrepreneurs which follows the journey of a group of Scots trying to launch their own businesses.
Mum-of-one Lisa joined ESpark's Glasgow hatchery last August and now works with almost 60 dogs.
Lisa said: "It's surreal, but also fantastic that we'll be on the big screen.
"In all the times I've been to the cinema, never did I think my wee dog walking firm would feature.
"I'm delighted that E-Spark felt we had done something right to merit the award and it allows me to build the business even further."
Stardust Dog Walking, based in Roybroyston, provide dog walking, dog day care and home boarding services in the North of Glasgow.
Lisa started the business in September 2012 and began trading in January 2013.