John Clark, 22, is alleged to have killed a 22-year-old sex offender in his cell at Barlinnie prison in Glasgow's East End.
Clark appeared in private yesterday at Glasgow Sheriff Court where he made no plea or declaration and was remanded in custody.
He will appear for a full committal hearing next week.
The body of the man was found by prison officers at the jail at around 2am on March 20.
The prisoner was found in his cell in E Hall on the fourth floor of the jail.