The campaign, run at the UK Border Agency office in Brand Street, Govan, uses posters with the slogan: "Ask about going home."
Public figures including Labour's Malcolm Chisholm and Nina Baker of the Green Party joined other opponents of the pilot outside the Brand Street offices.
Demonstrators held posters saying: "We belong to Glasgow," and "Justice, freedom and dignity for all."
The controversial pilot scheme, being run in the city, started on July 29 and runs until October 4.
A spokeswoman from Positive Action said: "'Go home' is a well-known racist taunt that has been used for decades in this country by fascists and racists against those of us from immigrant communities.
"That a government agency should decide to take up the same racist and xenophobic refrain while "processing" would-be refugees to this country, is shameful and deeply offensive."