Network Rail is reportedly considering a complete shutdown of Queen Street station in Glasgow for as much as a year during upgrading works.
They will include lengthening platforms to accommodate longer electric-powered trains and the installation of overhead electrical wires.
The rebuild is due to happen between August 2015 and December 2016.
Queen Street is used by 21 million passengers a year and is particularly busy with commuters using the express service to Edinburgh.
Network Rail rejected reports that the station could close for a full year, but acknowledged that closure is one option being considered.
A spokesman stressed a complete shutdown was "no more likely than anything else at this stage".
He said: "We are currently working with Transport Scotland to plan the Edinburgh to Glasgow Improvement Programme and, quite rightly, a variety of different options are being considered."
Stuart Patrick, Glasgow Chamber of Commerce chief executive, called on rail bosses to examine all possibilities before coming to an agreement.
He said: "We would be encouraging them to look at the full range of options before making a decision as it does seem drastic to close a station of such importance as Queen Street for a year."
The lengthening of the station platforms will require the demolition of part of the Millennium Hotel, next to the station.