Rhythm Base, which was launched in 2006 by two drummers, last night won the Evening Times-backed accolade at the biggest-ever Glasgow Business Awards.
The firm beat much-loved family business Station Flowers, popular bar and restaurant WEST brewery and top sandwich takeaway Where the Monkey Sleeps, after a public vote.
Glasgow businessmen Paul Burke, 38, and Gordon Ferguson, 34, set up Rhythm Base, in Commerce Street, because of their love of drumming – and because they could not find any percussion specialists in the city.
They have made a name for themselves in the music world, with famous visitors including the drummers for pop stars Pink and Paul Weller.
The pair also hold drumming workshops for people of all levels to come in and try out the percussion instruments on the premises.
Paul Burke said: "We were just delighted to be nominated so it's a shock to be winners.
"It's fantastic to be in this category with established businesses like WEST Brewery.
"It's a credit to all our five staff and it's great they were able to come and celebrate with us at the awards night.
Evening Times editor Tony Carlin, who presented the award, said: "The Evening Times is delighted once again to have been media partner for the Glasgow Business Awards.
"All of our finalists were exceptional and all make a remarkable contribution to Glasgow's business success story.
"Congratulations to our winners, Rhythm Base, chosen by our readers.
"They have all the positive qualities you would expect of Glasgow's favourite business and fully deserve this accolade."
The business was one of a host of well-deserved winners recognised at the glamorous ceremony held at the Thistle Glasgow Hotel.
A Scottish aviation expert, a chocolate specialist and a film festival were all honoured in front of more than 600 guests as DJ Des Clarke hosted the event.
Loganair's recently retired chairman Scott Grier was given the Chamber's exceptional Lifetime Achievement Award.
Mr Grier, who lives in West Kilbride, was honoured for his 36 years of service to the Glasgow-based company, heading up a management buy-out and building the airline from a staff of 44 to over 500, while increasing its fleet size to 26 planes.
The 71-year-old said winning the accolade was the "icing on the cake" to his long career.
He added: "It was quite well timed for me to be recognised like this, but it came as quite a surprise.
"It's fantastic to see some of the successes of the business community in Glasgow and to see how many companies are flourishing in the industry."
Glasgow-based renewables engineering consultancy SgurrEnergy walked away with the top accolade in two separate categories at the Chamber of Commerce organised event.
The firm was recognised by the event's sponsor, Bank of Scotland, as Most Outstanding Business overall while also taking the prize for International Trade.
Stuart Patrick, chief executive of Glasgow Chamber of Commerce, said the success of SgurrEnergy "reaffirms Glasgow's position as a genuine hub for renewable energy research and development".
Specialist chocolate company Nucoco, based in Govan, also scooped a top award at the annual event, now in its 15th year.
Jackie Wynne, 40, from Airdrie, launched Nucoco 18 months ago.
She said: "I'm absolutely over the moon. We're just a start-up business so it's a real surprise to be recognised like this."
The firm, which specialises in handcrafted confectionery, picked up the award for Best Use of Social Media.
Other winners included Glasgow Film Festival for best Creative Marketing and Glasgow Airport for Excellence in Customer Service.
Mr Patrick said the awards were the biggest yet.
He said: "Across all sectors, businesses in Glasgow have helped to make these awards a major success, attracting a record number of entries – a 75% increase on 2011 – which reflects the strength, confidence and diversity of the city's business community."
Evening Times Award for Glasgow's Favourite Business: Rhythm Base