Bosses of Samuel Dow in Nithsdale Road have applied for planning permission to create a fire exit to the rear of the building and escape doors to the front.
But the scheme has resulted in 50 objections, including one from SNP Deputy Leader and Glasgow Southside MSP Nicola Sturgeon.
The Strathbungo Society and the Architectural Heritage Society has also objected to the plan which officials have recommended gets the go-ahead.
Locals are worried the work will result in neighbouring properties suffering significant extra noise and that smokers will congregate in one area increasing noise, smoke pollution and litter.
They are also worried the fire escape stair would provide views into neighbouring homes and gardens.
Sammy Dow's is sited on the west side of Nithsdale Road and is an extended ground floor property at the end of a late 19th century Georgian terrace of tenements.
The site is category B listed and lies within the Strathbungo conservation area.
A report to councillors says: "This frontage is in a prominent position within an established commercial area of the conservation area.
"The proposed works to the front elevation are considered to be acceptable and will not affect the architectural character of the property.
"The multi-paned windows are not original and although a recognisable feature of the premises, their replacement with fixed clear glazing in a timber frame is acceptable.
"The internal works will upgrade facilities and would not affect the character of the property.
"It is appropriate for an external fire exit stair to be located to the rear of a property and in this instance, the stair will be fixed to a later extension of the property and located to the rear.
"The addition of the external stair is therefore not of concern in terms of the character of the listed building or the surrounding conservation area."
Planners have welcomed the scheme but admit adding a new fire escape to the rear could result in the capacity of the pub being increased.
The application is due to be decided by the city council planning committee on Tuesday.