The Geordie duo, Anthony McPartlin and Declan Donnelly, will perform at the SSE Hydro on Tuesday, August 26 2014.
The pair announced they would be taking the show on the road at a press conference at the O2 in London today.
The 2014 tour will also visit London, Cardiff, Manchester, Belfast and Newcastle.
The shows will include features from the television version of the show, including celebrity guests, the Super Computer and Little Ant and Dec.
However, Donnelly said "no two shows will be the same" and "the audience will take centre stage".
The pair, who first got together as child actors on Byker Grove, said they were not worried about getting on each other nerves during a long nationwide tour.
McPartlin said: "If we're not fed up of each other now after all these years, we've lived together, we still live four doors away from each other, I don't think these cities will bother us".
Tickets for the show go on sale at 9am on Friday, November 8.