Emergency services were called to the A77 near the junction with Maybole Road in Ayr, South Ayrshire at 6.45pm yesterday.
The young man was riding his black and orange KTM motorcycle north on the A77 when he was in a crash with a grey Volvo and silver Citroen which were both travelling south, police said.
The 23-year-old died at the scene.
The 51-year-old female driver of the Volvo and the 66-year-old man driving the Citroen were not injured.
Pc Michael Prout of Police Scotland has issued an appeal for witnesses to call Irvine police office on 01294 404 400 or the non-emergency number 101, or Crimestoppers anonymously on 0800 555 111.