Speaking at the Liberal Democrats' Conference in Glasgow, Scottish Secretary Michael Moore said identity was multi-layered and only the SNP want people to make a choice.
Mr Moore said he had many different identities, reflecting his many personal and professional roles in life, and insisted "true Scots" can back the Union.
He was speaking at a fringe event on identity at the party's UK conference, held at SECC.
He said: "I'm Scottish, British and European.
"In the same way that I am a husband, father, son, accountant, Liberal Democrat.
"In today's world our identity is complex and multi-layered.
He said there were concerns the SNP would attempt to focus the latter stages of the referendum campaign on identity.
He said: "If any nationalist in this debate says that people must choose between Scotland and the rest of the UK, our clear response will be this: No we don't. We are both Scottish and British, we are comfortable with both, we benefit from both, and there is no need to choose.
"Only nationalists ask that we make that choice.
"We need not make ourselves less than we are."
Today, Business Secretary Vince Cable is expected to tell conference he is seeking ways to ensure the National Minimum Wage can rise by more than rules allow at present.
He is also expected to call for tougher action on rogue company directors, who, he believes, get off too lightly for financial misdemeanours.
He will say: "We need to see fairness as well as trust in our director disqualification regime.
"For too long, a small rotten core has got away with either a slap on the wrist, a ban from working in their own industry or, at the most, a time-limited ban.
"I will beef up laws to ban rogue directors from running British companies, so dodgy directors face the strongest possible consequences for their irresponsible actions."
Mr Cable is also set to seek measures to prevent abuses from zero-hours work contracts, saying: "I am determined to make sure people are paid and treated fairly, while helping to keep people employed in these delicate economic times."