Youngsters wearing trainers and yellow baseball caps descended on the Glasgow park to raise money for the Prince & Princess of Wales Hospice.
The P5, P6 and P7 pupils walked the three-mile route before taking part in a series of games and sports activities, including football, rugby, golf, climbing, cheerleading and netball.
The 10th annual event, which was sponsored by Robert Wiseman Dairies, drew participants from 54 primary schools across the city.
The location had added resonance this year as the route passed the site that could be where a new-build hospice is constructed to replace the existing Georgian terrace in Carlton Place, Gorbals.
The Evening Times is backing plans to create a £15million facility on the eight-acre site, which would extend the hospice's existing services by having single-roomed accommodation and providing specialist palliative care for people aged 15-25.
Hospice community fundraiser Joyce Ross said: "We visited most of the schools before we came here so the children know why they are doing it – it is very important they know they are making a difference.
"This is a big thank you to the pupils for the sponsorship money they are raising."
Last year's event attracted 2300 participants, raising almost £16,000. Organisers will find out today how much this year's event raised.
Six pupils from Hillington Primary created their own Go Yellow T-shirts for the occasion.
Amy Neil, 12, said: "We thought of doing something different, so we decided to make a T-shirt.
"It felt like the walk was going to be longer, but it was fun."
Some 200 pupils from St Cadoc's Primary, Newton Mearns, joined in the event, which aims to foster good relations between schools.
Pupil support teacher Victoria Slater said: "They are so excited to come back, especially the primary sevens, who were here last year. It is a great atmosphere.
"We do a lot of twinning events with other schools, so we are used to working with other children and it is something they are very positive about."
Hundreds of youngsters queued up for the inflatable assault course of The Gladiator Programme and danced with Drumchapel Gymnastics Club.
In addition to Go Yellow's fun activities, walkers were treated to rides on a cart drawn by Clydesdale horses and met Pollok Park's newborn Highland calf, Uallach.
Strathclyde Fire & Rescue illuminated youngsters on the dangers of fire, with two dramatic 'Thermal Blast' demonstrations.
Nine colourful mascots, including Harvey from the hospice, Rangers' Broxi, Celtic's Hoopy, Safe-T Rex, Active Amy, Real Radio's Barry The Bassett Hound, the Gladiagator from The Gladiator Programme and Wanda from Wiseman Dairies took part in a 30-metre dash before taking to the stage for a mass 'Cha Cha Slide'.
Sandy Wilkie, of Wiseman Dairies, said: "We are delighted to be able to contribute to such a great day out for local children."
The yellow theme continued in the goodie bags, as Cordia, the catering company for the council, donated 3000 bananas for the event.
The hospice has to raise £2.8million annually from fundraising and voluntary donations. Go Yellow originated from the Help The Hospices appeal, which organises a series of yellow-themed events every June.