The Light Blues Under-20s went down 2-1 to Motherwell at Ibrox as they missed the chance to close the gap on top-of-the- table Hamilton Accies.
Boss Durie fielded first-team stars Steve Simonsen, Dean Shiels and David Templeton for the Steelmen clash, with the former Hearts star pulling the Gers level.
Kyle McAusland, Luca Gasparotto, Barrie McKay and Fraser Aird also featured for the Ibrox second string and Durie is keen for his players to get back to winning ways.
He said: "We've had a couple of bad results now and (Tuesday) night was really disappointing.
"We can't afford to give away goals like we did and expect to win games.
"The boys are putting themselves up against it right away with the calibre of goals they are losing and both against Motherwell were from set-plays. That is disappointing.
"Teams playing against us aren't creating many chances but they're scoring from set-plays and that's massive.
"We did create a lot more and in the final third we should perhaps have done a bit better so all in all, it was a disappointing night.
"The players have got to learn. We told the players before the game not to give away silly free-kicks then Motherwell got both their goals from silly fouls.
"The players have to realise that if they do that they'll get punished and that's unfortunately what happened (on Tuesday). If you lose goals like that, you don't deserve to win games."
After starting their Under-20 league campaign in fine form, the Rangers second string have now slipped to consecutive defeats following their loss to Aberdeen last week.
Goals from Craig Moore and Dale Shirkie handed Jonatan Johannson's side the win at Ibrox and Durie is confident his side can pick up their performance levels again.
"I don't think we've started and there's a lot more to come from us as a team," Durie told rangers.co.uk.
"If we cut out our mistakes and are a bit more incisive in the final third, I think we'll come good.
"Our players are here because they're good players. They're going to make mistakes but it's important they learn."