The Gers Nine-in-a-Row star expects a hot reception from Ally McCoist's men who will be facing SPL opposition for the first time this term since their rebirth in the Third Division.
With Motherwell currently sitting top of the SPL, unbeaten in all nine outings this term, McCall has no doubt that Rangers will have an added incentive to extend their winning run at Ibrox over Well which goes back to 1997.
He said: "I think that both the players and staff at Rangers have taken a kicking since their defeat to Queen of the South.
"Now we are rolling up as the first SPL opposition they will have faced this season and it is a great opportunity for them to respond to all of that.
"I think it might also be a plus for Rangers that they are not going into this one as massive favourites, which they have done in every game so far this season.
"When you factor in that and that the home crowd at Ibrox will be roaring them on as a 12th man to take out an SPL side and prove a point, it probably is the worst tie we could have got and at the worst time in respect of going to Ibrox."
But McCall, who will have a full complement to call on, believes that his men have grown in confidence in recent weeks and will take belief from a 0-0 draw they earned in their last trip to Govan back in May which was also Gers' last meeting with an SPL side on home soil.
He said: "I said to the boys after that one that when we next came to Ibrox we must draw upon the experience and lessons we learned in that one and put it to best use.
"Now we have that opportunity but not in the context I expected."
Despite the fact his side lost a two-goal lead at Aberdeen on Sunday to draw 3-3, McCall says his men will head to Ibrox full of confidence and self-belief.
He said: "To be honest I think this is a tie that neither ourselves or Rangers would have wanted. Certainly it is the toughest we could have had.
"But our form has been good and consistent and this is a big test of where we are.
"Rangers may have lost several top class internationals but they have not done too badly with the lads they have recruited.
"So we will need to be at our best to end our long losing run at Ibrox. But it is a tie we are all looking forward to and a challenge we are up for."