The interim Scotland boss has selected nine of the new starts from the Glasgow ranks with Ryan Wilson, Chris Fusaro, Sean Kennedy, Pat MacArthur, Sean Maitland, Peter Murchie, Tommy Seymour, Alex Dunbar and Peter Horne, all receiving recognition for the first time.
In all, there are 19 players from the Gregor Townsend's squad with the squad completed by nine exiles and seven from Edinburgh Rugby.
Johnson said: "We've chosen this squad with two parameters uppermost in our minds and they are current form and looking to the future. Where two players have been vying for the one spot in areas such as the back-row, we've gone with the form option.
"I've been impressed with a number of the young fellows who've helped Glasgow to second place in the Rabo, especially by their work ethic and determination to improve.
"This squad is for our training camp in Glasgow next week and we still have the option to add to it for the Six Nations."
The inclusion of 21-year-old scrum-half Kennedy, a product of the Alloa and Stirling County clubs who started the season as an elite development player with Edinburgh Rugby before being sent to Glasgow, caps a meteoric rise.
The recall of former Glasgow No.8 Johnnie Beattie, who is now at Montpellier, is a fascinating call by Johnson.
Scotland Training squad: Backs: Murchie, Hogg, Maitland, Seymour, Lamont (all Glasgow Warriors), Visser (Edinburgh Rugby), Evans (Castres), Scott (Edinburgh Rugby), Dunbar, Horne, Weir, Jackson (all Glasgow Warriors), Heathcote (Bath Rugby), Pyrgos, Kennedy (both Glasgow Warriors), Greig Laidlaw (Edinburgh Rugby).
Forwards: Dickinson (Sale Sharks), Grant, Hall, MacArthur (all Glasgow Warriors), Ford (Edinburgh Rugby), Murray (Worcester Warriors), Cross (Edinburgh Rugby), Low, Kellock (both Glasgow Warriors), Gray (Sale Sharks), Hamilton (Gloucester), Gilchrist (Edinburgh Rugby), Brown (Saracens), Harley, Wilson (both Glasgow Warriors), Beattie (Montpellier), Denton (Edinburgh Rugby), Vernon (Sale Sharks), Fusaro (Glasgow Warriors).