Coach Andy Robinson has selected Stuart Hogg, the 19-year-old full-back and his team-mates, stand-off Duncan Weir, 20 and flanker/lock Rob Harley, 21.
Another three uncapped players were selected along with all 28 available players from last year's Rugby World Cup squad, following the retirements of Chris Paterson and Nathan Hines.
The other three rookies are 23-year-old Edinburgh wing Lee Jone, Sale Sharks' former Edinburgh and Dunfermline flanker/lock Fraser McKenzie, also 23, and 20-year-old London Irish centre Steven Shingler.
Shingler, born in Swansea and who joined London Irish from Scarlets at the start of this season, qualifies for Scotland through his Dumfries-born mum.
Robinson said: "I am pleased to reflect on the winning displays we have seen from both Edinburgh Rugby and Glasgow Warriors over the last two months and the contribution a number of our uncapped players have made to them."
Backs – Joe Ansbro (London Irish), Mike Blair (Edinburgh Rugby), Chris Cusiter (Glasgow Warriors), Simon Danielli (Ulster), Nick De Luca (Edinburgh Rugby), Max Evans (Castres), Stuart Hogg, Ruaridh Jackson (both Glasgow Warriors), Lee Jones, Greig Laidlaw (both Edinburgh Rugby), Rory Lamont (Glasgow Warriors), Sean Lamont (Scarlets), Rory Lawson (Gloucester), Graeme Morrison (Glasgow Warriors), Dan Parks (Cardiff Blues), Steven Shingler (London Irish) and Duncan Weir (Glasgow Warriors).
Forwards – John Barclay (Glasgow Warriors), Kelly Brown (Saracens), Geoff Cross, David Denton (both Edinburgh Rugby), Alasdair Dickinson (Sale Sharks), Ross Ford (Edinburgh Rugby), Richie Gray, Dougie Hall (both Glasgow Warriors), Jim Hamilton (Gloucester), Robert Harley (Glasgow Warriors), Allan Jacobsen (Edinburgh Rugby), Alastair Kellock (Glasgow Warriors), Scott Lawson (Gloucester), Moray Low (Glasgow Warriors), Fraser McKenzie (Sale Sharks), Euan Murray (Newcastle Falcons), Ross Rennie (Edinburgh Rugby), Alasdair Strokosch (Gloucester) and Richie Vernon (Sale Sharks).